Preface

Contributors

In Vitro Analysis of GPI Biosynthesis in Mammalian Cells

Victoria L. Stevens

Selection of Mammalian Cell Mutants in GPI Biosynthesis

Victoria L. Stevens

Analysis of the Cell-Surface Distribution of GPI-Anchored Proteins

Satyajit Mayor

Imaging Fluorescence Resonance Energy Transfer as Probe of Membrane Organization and Molecular Associations of GPI-Anchored Proteins

Anne K. Kenworthy

Michael Edidin

Purification of Caveolae-Derived Membrane Microdomains Containing Lipid-Anchored Signaling Molecules, Such as GPI-Anchored Proteins, H-Ras, Src-Family Tyrosine Kinases, eNOS, and G-Protein α-, β-, and γ-Subunits

Michael P. Lisanti

Massimo Sargiacomo

Philipp E. Scherer

Analysis of Lipids in Caveolae

Jun Liu

Jan E. Schnitzer

Analysis of the Carbohydrate Components of Glycosylphosphatidylinositol Structures Using Fluorescent Labeling

Nicole Zitzmann

Michael A. J. Ferguson

Analysis of the Lipid Moiety of GPI Anchor in the Yeast Saccharomyces cerevisiae

Fulvio Reggiori

Elisabeth Canivenc-Gansel

Andreas Conzelmann

Rapid Identification of Cysteine-Linked Isoprenyl Groups by Metabolic Labeling with [3H]Farnesol and [3H]Geranylgeraniol

Douglas A. Andres

Dean C. Crick

Brian S. Finlin

Charles J. Waechter

Incorporation of Radiolabeled Prenyl Alcohols and Their Analogs into Mammalian Cell Proteins: A Useful Tool for Studying Protein Prenylation

Alberto Corsini

Christopher C. Farnsworth

Paul McGeady

Michael H. Gelb

John A. Glomset

Reconstitution of Yeast Farnesyltransferase from Individually Purified Subunits

Jun Urano

Fuyuhiko Tamanoi

Probing the Role of H-Ras Lipidation for Signaling Functions in Xenopus laevis Oocytes

Thomas Dudler

Michael H. Gelb

Fluorescence Measurement of Lipid-Binding Affinity and Interbilayer Transfer of Bimane-Labeled Lipidated Peptides

John R. Silvius

Determination of the Kinetics of Intervesicle Transfer and Transbilayer Diffusion of Bimane-Labeled Lipidated Peptides

John R. Silvius

Preparation and Assay of Myristoyl-CoA: Protein N-Myristoyltransferase

Rajala V. S. Raju

Rajendra K. Sharma

Metabolic Labeling of Protein-Derived Lipid Thioesters in Palmitoyl-Protein Thioesterase-Deficient Cells

Sandra L. Hofmann

Jui-Yun Lu

Fatty Acid Analysis of Protein-Derived Lipid Thioesters Isolated from Palmitoyl-Protein Thioesterase-Deficient Cells

Sandra L. Hofmann

Linda A. Verkruyse

Index