Preface

Introduction

Questions about the Behaviour of Bacterial Pathogens in Vivo

DNA Topology and Adaptation of Salmonella typhimurium to an Intracellular Environment

New Methods for Studying Bacterial Behaviour in Vivo

The Pathogenesis of Shigella flexneri Infection: Lessons from in Vitro and in Vivo Studies

Detection and Analysis of Gene Expression During Infection by in Vivo Expression Technology

Measurement of Bacterial Gene Expression in Vivo

Identification and Analysis of Bacterial Virulence Genes in Vivo

Salmonella Interactions with Host Cells: In Vitro to in Vivo

Impact of the New Methods

In Vivo Gene Expression and the Adaptive Response: From Pathogenesis to Vaccines and Antimicrobials

Challenge of Investigating Biologically Relevant Functions of Virulence Factors in Bacterial Pathogens

Virulence Gene Regulation Inside and Outside

Evidence for Operation in Vivo of Aspects of Pathogenicity Revealed by Recent Work in Vitro: Potential Use of New Methods

Quorum Sensing and the Population-dependent Control of Virulence

Type III Secretion: A Bacterial Device for Close Combat with Cells of Their Eukaryotic Host

Evolution of Microbial Pathogens

The Immune Responses to Bacterial Antigens Encountered in Vivo at Mucosal Surfaces

Index