search for books and compare prices
Tables of Contents for Lectin Histochemistry
Chapter/Section Title
Page #
Page Count
Abbreviations
ix
4
Preface
xiii
1. An Introduction to the Field
1
18
Definition
1
1
A brief history of lectinology
2
1
Nomenclature
3
2
Simple and complex sugars
5
3
Sugar specificity of lectins
8
4
Sources and occurrence
12
1
Detection
13
1
Lectin structure
14
1
Functions of lectins
15
1
What can lectins be used for?
16
1
Why use lectins?
16
1
References
17
2
2. Sources of Lectins and Other Reagents for Histochemistry
19
26
Isolation of lectins from plant material
19
12
Commercial sources of lectins and other reagents
31
6
How to make your own polyclonal antibodies against lectins
37
3
An alternative to using antibodies: labelled lectins
40
2
References
42
3
3. Lectin Histochemistry for Light Microscopy: I. Principles, Sample Preparation and Choice of Label
45
30
Definition of lectin histochemistry
45
1
A philosophical point before starting
45
1
Basic requirements for successful lectin histochemistry
46
1
Preparation of specimens: cells and tissues
46
15
Labels for detection of lectin binding
61
12
Reference
73
2
4. Lectin Histochemistry for Light Microscopy: II. Methods for Visualization of Lectin Binding
75
24
General practical notes
75
5
Direct method
80
3
Simple indirect method
83
2
APAAP or PAP methods
85
1
Avidin-biotin methods
86
5
A word about kits
91
1
Controls
91
3
Some ideas on choice of lectins
94
1
Hazardous lectins
94
1
Which tissues to use
94
1
Foolproof methods for the beginner
95
1
The way forward: characterization of complex carbohydrates
96
2
References
98
1
5. Electron Microscopic Methods for Demonstration of Lectin Binding Sites
99
10
Introduction
99
1
Choice of label
99
1
Direct and indirect detection methods
100
1
Pre-embedding and post-embedding techniques
101
2
Methods for demonstration of lectin binding at the EM level
103
2
Controls
105
2
References
107
2
6. Analysis of Glycoproteins by Electrophoretic Techniques with Western Blotting using Lectins
109
22
Limitations of electrophoretic techniques
109
1
Sample preparation
110
3
Preliminary analysis of lectin binding by dot-blotting
113
1
Analysis of glycoproteins by SDS-PAGE and Western blotting using lectins
114
12
Analysis of glycoproteins by IEF and lectin blotting
126
3
References
129
2
7. Interpretation and Analysis of Lectin Binding
131
6
Interpretation of lectin binding to tissue sections: simple and complex sugars
131
1
Lectin binding to a range of binding partners
132
1
Patterns of binding to cells and tissues
132
2
Recording staining results
134
2
Analysis of lectin binding partners
136
1
References
136
1
8. Applications and Usefulness of Lectin Binding Studies
137
14
Strategy for use of lectin histochemistry
137
3
Mapping lectin binding to normal tissues
140
2
Identification of cell lineage
142
1
Mapping lectin binding in disease
142
2
Other approaches
144
1
References
145
6
9. Histochemistry to Localize Endogenous Lectins
151
8
Introduction
151
2
Cellular functions of glycoconjugates
153
1
Endogenous lectins
153
1
Localization of endolectins by in situ hybridization
153
1
Localization of endolectins by conventional immunohistochemisty
154
1
Localization of endolectins through binding of labelled neoglycoproteins
154
3
References
157
2
Appendices
159
10
A: Recipes
159
8
B: Suppliers
167
2
Index
169