Preface

Contributors

Target Format and Hybridization Conditions

Alex Reid

End Labeling Procedures: An Overview

Elena Hilario

Photobiotin Labeling

Elena Hilario

Nick Translation

Alex Reid

Random Primed Labeling

Alex Reid

Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ Hybridization

Philip Hugenholtz

Gene W. Tyson

Linda L. Blackall

Evaluation of Alterations in the Tumor Suppressor Genes INK4A and INK4B in Human Bladder Tumors

Irene Orlow

Carlos Cordon-Cardo

The β-Tubulin Gene Region as a Molecular Marker to Distinguish Leishmania Parasites

Alexis Mendoza-Leon

Luis Luis

Clara Martinez

Detection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple Myeloma

Ross D. Brown

P. Joy Ho

Detection of Chlamydia trachomatis by DNA Amplification

Peter Timms

Sarah Mathews

Isolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene Probes

Alex van Belkum

Nicole van den Braak

Leen-Jan van Doorn

Hubert Endtz

Diagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain Reaction

Gerard J. Nuovo

Quantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA Probes

Teresa Bisucci

Tim D. Hewitson

Ian A. Darby

Stable Isotope Probing for Detection of Methanotrophs After Enrichment with 13CH4

Stefan Radajewski

J. Colin Murrell

Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes)

Gillian D. Lewis

Sally A. Anderson

Susan J. Turner

Analysis of 16S--23S rRNA Intergenic Spacer of Vibrio cholerae and Vibrio mimicus for Detection of These Species

Jongsik Chun

Irma N. G. Rivera

Rita R. Colwell

Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes

Laura G. Leff

In Situ Hybridization of Burkholderia cepacia Cells

Christopher J. McNamara

Laura G. Leff

Detection of Polychlorinated Biphenyl-Degrading Organisms in Soil

John Sanseverino

Alice C. Layton

Gary S. Sayler

Detection of Single-Copy Genes in DNA from Transgenic Plants

Lee C. Garratt

Matthew S. McCabe

J. Brian Power

Michael R. Davey

Differentiation Between Transcripts of Genes Belonging to Small Families During Fruit Ripening and Abscission

Claudio Bonghi

Benedetto Ruperti

Pietro Tonutti

Polymerase Chain Reaction Detection of Invasive Shigella and Salmonella enterica in Food

Keith A. Lampel

Palmer A. Orlandi

Polymerase Chain Reaction for Detection of Listeria monocytogenes

Debra K. Winters

Index