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Tables of Contents for Drosophila Protocols
Chapter/Section Title
Page #
Page Count
Preface
ix
Abbreviations
xi
CHROMOSOMES
Preparation and Analysis of Drosophila Mitotic Chromosomes
3
22
S. Pimpinelli
S. Bonaccorsi
L. Fanti
M. Gatti
In Situ Hybridization to Somatic Chromosomes
25
32
A.F. Dernburg
BrdU Labeling of Chromosomes
57
10
A.W. Shermoen
Analysis of Meiosis of Fixed and Live Oocytes by Light Microscopy
67
20
H.J.G. Matthies
M. Clarkson
R.B. Saint
R. Namba
R.S. Hawley
Cytological Analysis of Spermatocyte Growth and Male Meiosis in Drosophila melanogaster
87
24
S. Bonaccorsi
M.G. Giansanti. G. Cenci
M. Gatti
Preparation and Analysis of Polytene Chromosomes
111
8
J.A. Kennison
In Situ Hybridization to Polytene Chromosomes
119
12
M.-L. Pardue
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
131
10
R. Paro
CELL BIOLOGY
Fluorescent Analysis of Drosophila Embryos
141
18
W.F. Rothwell
W. Sullivan
Imaginal Discs
159
16
S.S. Blair
The Neuromuscular Junction
175
26
H.J. Bellen
V. Budnik
Histological Techniques for the Drosophila Eye. Part I: Larva and Pupa
201
28
T. Wolff
Histological Techniques for the Drosophila Eye. Part II: Adult
229
16
T. Wolff
Preparation of Thin Sections of Drosophila for Examination by Transmission Electron Microscopy
245
28
K.L. McDonald
D.J. Sharp
W. Rickoll
Electrophysiological Approaches to the Neuromusculature
273
24
K.S. Broadie
Functional Assays of the Peripheral and Central Nervous Systems
297
16
K.S. Broadie
GFP and Other Reporters
313
32
T. Hazelrigg
Quantitative Microinjection of Drosophila Embryos
345
16
D.P. Kiehart
J.M. Crawford
R.A. Montague
Targeted Disruption of Gene Function in Drosophila by RNA Interference
361
12
L. Misquitta
B.M. Paterson
Drosophila Cell Culture and Transformation
373
16
L. Cherbas
P. Cherbas
Generating Antibodies against Drosophila Proteins
389
24
I. Rebay
R.G. Fehon
Photoactivated Gene Expression for Cell-fate Mapping and Cell Manipulation
413
16
J. Minden
R. Namba
S. Cambridge
MOLECULAR BIOLOGY
Recovery of DNA Sequences Flanking P-element Insertions: Inverse PCR and Plasmid Rescue
429
10
A.M. Huang
E.J. Rehm
G.M. Rubin
GAL4-mediated Ectopic Gene Expression in Drosophila
439
10
P. van Roessel
A.H. Brand
Functional Cell Ablation
449
30
S.T. Sweeney
A. Hidalgo
J.S. deBelle
H. Keshishian
GENOMICS
Preparation of DNA from E. coli Cells Containing Bacteriophage P1 Clones
479
8
E.R. Lozovskaya
Drosophila Microarrays: From Arrayer Construction to Hybridization
487
22
K.P. White
K.C. Burtis
Using Drosophila Genome Databases
509
16
S. Misra
M.A. Crosby
R.A. Drysdale
Preparation and Analysis of High-molecular-weight DNA in Drosophila
525
16
G.H. Karpen
BIOCHEMISTRY
Culturing Large Populations of Drosophila for Protein Biochemistry
541
12
J.C. Sisson
Preparation of Nuclear Extracts from Drosophila Embryos and In Vitro Transcription Analysis
553
10
M.J. Pazin
Preparation of Membrane Proteins from Drosophila Embryos
563
8
C.X. Zhang
T.-S. Hsieh
Preparing Cytoplasmic Extracts from Drosophila Embryos
571
6
M. Moritz
Immunoblotting of Proteins from Single Drosophila Embryos
577
8
T.T. Su
THE ORGANISM
Laboratory Culture of Drosophila
585
16
M. Ashburner
J. Roote
Preparation of Larval and Adult Cuticles for Light Microscopy
601
16
D.L. Stern
E. Sucena
Exposing Drosophila to Neuroactive Drugs
617
68
J. Hirsh
APPENDICES
1 1989 Table of Contents from Drosophila: A Laboratory Manual
625
10
2 Anatomical Drawings of Drosophila
635
20
3 Solutions and Reagents
655
6
4 Cautions
661
18
5 Suppliers
679
2
6 Trademarks
681
4
Index
685