search for books and compare prices
Tables of Contents for Monoclonal Antibodies
Chapter/Section Title
Page #
Page Count
Contributors
ix
Preface
xi
Glossary
xiii
Antibodies as laboratory and therapeutic reagents
1
16
H Zola
Introduction: antibody specificity and diversity
1
1
Antibody specificity: protein structure
2
1
The immunoglobulin constant region: form and functions
3
2
The cells that make antibody
5
1
The basis of antibody specificity and diversity: unique genetic mechanisms
6
2
Polyclonal antisera
8
1
Monoclonal antibodies
9
1
The major differences between monoclonal and polyclonal antibodies
10
2
Applications of antibodies in the laboratory
12
1
Therapeutic applications of antibodies
13
2
Improving on nature - antibody engineering
15
1
Replacing nature - antibodies from gene libraries
15
1
Conclusions
15
2
Hybridoma technology: making monoclonal antibodies
17
28
B Hunt
H Zola
C Goddard
Introduction
17
1
Theoretical basis
17
1
Cell culture
17
3
Screening assay
20
1
Immunization
21
2
Myeloma cells
23
1
Fusion protocol
24
1
Post-fusion care
24
2
Cloning
26
1
Cryopreservation
26
1
Specificity and isotyping
26
1
Mycoplasma
27
1
Large scale antibody production
27
2
Antibody purification
29
1
Storage and quality control
30
15
Screening hybridomas by enzyme-linked immunoassay (ELISA)
32
2
Conjugation of antigen to a carrier protein
34
1
Immunization with a soluble protein available in quantity
35
1
Fusion - standard protocol using polyethylene glycol (PEG)
36
3
Cloning of hybridoma cells
39
1
Cryopreservation of hybridoma cells
40
2
Purification of IgG1 on Protein G
42
3
Antibody engineering
45
36
MA Thiel
G Pilkington
H Zola
Introduction
45
1
Choice of antibody designs to suit specific applications
46
5
Chimeric and humanized whole antibodies
51
1
Single domain antigen binding fragments
52
1
Engineering antibody fragments
53
2
Expression of antibody fragments
55
2
Purification of scFv antibody fragments
57
1
Phage display
57
24
Preparation and selection of phage display library of Fab fragments
67
12
Purification of human Fab
79
2
Applications of monoclonal antibodies to the study of molecules in solution
81
20
DA Brooks
Introduction
81
2
ELISA (enzyme linked immuno-adsorbent assay)
83
3
Protein epitope delineation
86
1
Assays of antibody-bound antigen
87
2
Immunoaffinity chromatography
89
2
Western blotting
91
1
Immunoprecipitation and radiolabel synthesis/processing
92
1
Immuno-organelle binding assays
93
8
Immunoaffinity chromatography
95
2
Western blotting
97
2
Radiolabel-immunoprecipitation protocol
99
2
Applications of monoclonal antibodies in microbiology
101
26
T Kok
P Li
I Gardner
Introduction
101
1
Diagnosing infection
102
2
Viruses
104
4
Mycoplasma and chlamydia
108
2
Applications in bacteriology
110
11
Parasitic and fungal infections
121
6
Detection of Neisseria meningitidis antigen by agglutination of monoclonal antibody-coated latex particles
124
3
Applications of monoclonal antibodies to the study of cells and tissues
127
20
H Zola
Introduction
127
1
The complexity of cells: the human CD antigens illustrate the heterogeneity of cellular composition
128
2
Cell markers: identification and enumeration of specific cell types
130
9
Antibody-based cell separation
139
2
Antibody-based cell destruction or agglutination
141
1
Analysis of function
141
1
Tissue analysis
142
5
Direct immunofluorescence
144
3
Index
147