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Tables of Contents for Methods in Enzymology
Chapter/Section Title
Page #
Page Count
CONTRIBUTORS TO VOLUME 296
ix
6
PREFACE
xv
2
VOLUMES IN SERIES
xvii
 
Section I. Purification of Transporter Proteins and cDNAs
3
162
A. Plasma Membrane Carriers
1. Purification, Hydrodynamic Properties, and Glycosylation Analysis of Glycine Transporters
3
14
CARMEN ARAGON
BEATRIZ LOPEZ-CORCUERA
2. Expression Cloning Using Xenopus laevis Oocytes
17
35
MICHAEL F. ROMERO
YOSHIKATSU KANAI
HIROMI GUNSHIN
MATTHIAS A. HEDIGER
3. Cloning of Genes or cDNAs Encoding Neurotransmitter Transporters and Their Localization by Immunocytochemistry
52
12
NATHAN NELSON
QING-RONG LIU
B. Vesicular Carriers
4. Purification of Vesicular Monoamine Transporters: From Classical Techniques to Histidine Tags
64
9
RODRIGO YELIN
SHIMON SCHULDINER
5. Noncovalent and Covalent Labeling of Vesicular Monoamine Transporter with Tetrabenazine and Ketanserin Derivatives; Purification of Photolabeled Protein
73
11
JEAN-PIERRE HENRY
CORINNE SAGNE
MARIE-FRANCOISE ISAMBERT
BRUNO GASNIER
6. Functional Identification of Vesicular Monoamine and Acetylcholine Transporters
84
15
HELENE VAROQUI
JEFFREY D. ERICKSON
7. Photoaffinity Labeling of Vesicular Acetylcholine Transporter from Electric Organ of Torpedo
99
17
STANLEY M. PARSONS
GARY A. ROGERS
LAWRENCE M. GRACZ
8. Bioenergetic Characterization of y-Aminobutyric Acid Transporter of Synaptic Vesicles
116
9
JOHANNES W. HELL
REINHARD JAHN
9. Solubilization and Reconstitution of Synaptic Vesicle Glutamate Transport System
125
19
SCOTT M. LEWIS
TETSUFUMI UEDA
10. Analysis of Neurotransmitter Transport into Secretory Vesicles
144
21
J. PATRICK FINN III
ANDREW MERICKEL
ROBERT H. EDWARDS
Section II. Pharmacological Approaches and Binding Studies
165
68
11. Inhibitors of y-Aminobutyric Acid Transport as Experimental Tools and Therapeutic Agents
165
10
POVL KROGSGAARD-LARSEN
BENTE F. FROLUND
ERIK FALCH
12. Design and Synthesis of Conformationally Constrained Inhibitors of High-Affinity, Sodium-Dependent Glutamate Transporters
175
14
A. RICHARD CHAMBERLIN
HANS P. KOCH
RICHARD J. BRIDGES
13. Examination of Glutamate Transporter Heterogeneity Using Synaptosomal Preparations
189
14
MICHAEL B. ROBINSON
14. Specificity and Ion Dependence of Binding of GBR Analogs
203
16
AMADOU T. CORERA
JEAN-CLAUDE DO REGO
JEAN-JACQUES BONNET
15. Cocaine and GBR Photoaffinity Labels as Probes of Dopamine Transporter Structure
219
14
ROXANNE A. VAUGHAN
Section III. Transport Assays and Kinetic Analyses
233
60
16. Ion-Coupled Neurotransmitter Transport: Thermodynamic vs. Kinetic Determinations of Stoichiometry
233
15
GARY RUDNICK
17. Inhibition of [3^H]Dopamine Translocation and [3^H]Cocaine Analog Binding: A Potential Screening Device for Cocaine Antagonists
248
11
MAARTEN E. A. REITH
CEN XU
F. IVY CARROLL
NIAN-HANG CHEN
18. Transport and Drug Binding Kinetics in Membrane Vesicle Preparation
259
19
HEINZ BONISCH
19. Use of Human Placenta in Studies of Monoamine Transporters
278
15
VADIVEL GANAPATHY
PUTTUR D. PRASAD
FREDERICK H. LEIBACH
Section IV. Biochemical Approaches for Structure-Function Analyses
293
132
20. Analysis of Transporter Topology Using Deletion and Epitope Tagging
293
14
JANET A. CLARK
21. Selective Labeling of Neurotransmitter Transporters at the Cell Surface
307
11
GWYNN M. DANIELS
SUSAN G. AMARA
22. Transmembrane Topology Mapping Using BiotinContaining Sulfhydryl Reagents
318
13
REBECCA P. SEAL
BARBARA H. LEIGHTON
SUSAN G. AMARA
23. Probing Structure of Neurotransmitter Transporters by Substituted-Cysteine Accessibility Method
331
16
JONATHAN A. JAVITCH
24. Biosynthesis. N-Glycosylation, and Surface Trafficking of Biogenic Amine Transporter Proteins
347
23
SAMMANDA RAMAMOORTHY
HALEY E. MELIKIAN
YAN QIAN
RANDY D. BLAKELY
25. Expression of Neurotransmitter Transport Systems in Polarized Cells
370
18
JINHI AHN
GRAZIA PIETRINI
THEODORE R. MUTH
MICHAEL J. CAPLAN
26. Localization of Transporters Using TransporterSpecific Antibodies
388
19
N.C. DANBOLT
K.P. LEHRE
Y. DEHNES
F.A. CHAUDHRY
L.M. LEVY
27. Generation of Transporter-Specific Antibodies
407
18
GARY W. MILLER
MICHELLE L. GILMOR
ALLAN I. LEVEY
Section V. Expression Systems and Molecular Genetic Approaches
425
126
28. Homologies and Family Relationships among Na^+/Cl^- Neurotransmitter Transporters
425
11
HOLGER LILL
NATHAN NELSON
29. Vaccinia Virus-T7 RNA Polymerase Expression System for Neurotransmitter Transporters
436
7
SUE L. POVLOCK
SUSAN G. AMARA
30. Baculovirus-Mediated Expression of Neurotransmitter Transporters
443
13
CHRISTOPHER G. TATE
31. Dopamine Transporter Mutants, Small Molecules, and Approaches to Cocaine Antagonist/Dopamine Transporter Disinhibitor Development
456
10
GEORGE UHL
ZHICHENG LIN
THOMAS METZGER
DALIT E. DAR
32. In Vivo Generation of Chimeras
466
9
KARI JOHNSON BUCK
SUSAN G. AMARA
33. Structural Determinants of Neurotransmitter Transport Using Cross-Species Chimeras: Studies on Serotonin Transporter
475
23
ERIC L. BARKER
RANDY D. BLAKELY
34. Molecular Cloning of Neurotransmitter Transporter Genes: Beyond Coding Region of cDNA
498
16
DAVID J. VANDENBERGH
35. Use of Antisense Oligodeoxynucleotides to Inhibit Expression of Glutamate Transporter Subtypes
514
15
LYNN A. BRISTOL
JEFFREY D. ROTHSTEIN
36. Using Caenorhabditis elegans to Study Vesicular Transport
529
22
JAMES B. RAND
JANET S. DUERR
DENNIS L. FRISBY
Section VI. Application of Electrophysiological Techniques to Neurotransmitter Carriers
551
98
37. Measurement of Transient Currents from Neurotransmitter Transporters Expressed in Xenopus Oocytes
551
15
SELA MAGER
YONGWEI CAO
HENRY A. LESTER
38. Fluorescence Techniques for Studying Cloned Channels and Transporters Expressed in Xenopus Oocytes
566
12
ALBERT CHA
NOA ZERANGUE
MICHAEL KAVANAUGH
FRANCISCO BEZANILLA
39. Fluctuation Analysis of Norepinephrine and Serotonin Transporter Currents
578
15
LOUIS J. DEFELICE
AURELIO GALLI
40. Serotonin Transport in Cultured Leech Neurons
593
15
DIETER BRUNS
41. Glutamate Uptake in Purkinje Cells in Rat Cerebellar Slices
608
9
MICHIKO TAKAHASHI
MONIQUE SARANTIS
DAVID ATTWELL
42. Patch-Clamp, Ion-Sensing, and Glutamate-Sensing Techniques to Study Glutamate Transport in Isolated Retinal Glial Cells
617
15
BRIAN BILLUPS
MAREK SZATKOWSKI
DAVID ROSSI
DAVID ATTWELL
43. Measurement of Glial Transport Currents in Microcultures: Application to Excitatory Neurotransmission
632
17
STEVEN MENNERICK
CHARLES F. ZORUMSKI
Section VII. Microdialysis and Electrochemical Measurements
649
82
44. Voltammetric Studies on Kinetics of Uptake and Efflux at Catecholamine Transporters
649
11
KRISSTINA DANEK
JAY B. JUSTICE, JR.
45. Resolution of Biogenic Amine Transporter Kinetics by Rotating Disk Electrode Voltammetry: Methodology and Mechanistic Interpretations
660
15
CYNTHIA EARLES
HOLLIE WAYMENT
MITCHELL GREEN
JAMES O. SCHENK
46. Electrochemical Detection of Reverse Transport from Planorbis Giant Dopamine Neuron
675
14
BRIAN B. ANDERSON
ANDREW G. EWING
DAVID SULZER
47. Measuring Uptake Rates in Intact Tissue
689
19
MELISSA A. BUNIN
R. MARK WIGHTMAN
48. High-Speed Chronoamperometric Electrochemical Measurements of Dopamine Clearance
708
11
NANCY R. ZAHNISER
SHELLY D. DICKINSON
GREG A. GERHARDT
49. In Vivo Microdialysis for Measurement of Extracellular Monoamine Levels Following Inhibition of Monoamine Transporters
719
12
NIAN-HANG CHEN
MAARTEN E. A. REITH
AUTHOR INDEX
731
32
SUBJECT INDEX
763