PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

Product Description: The Condensed Protocols From Molecular Cloning: A Laboratory Manual is a single volume adaptation of the three volume third edition of Molecular Cloning: A Laboratory Manual. This condensed book contains only the step by step portions of the protocols, accompanied by selected appendices from the world s best selling manual of molecular biology techniques...read more

Product Description: Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies...read more

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Product Description: Distinguished scientists and researchers present a comprehensive collection of current preparative PCR techniques that can be used in cloning and modifying DNA and cDNA. Topics include performing and optimizing PCR (including long PCR), cloning PCR products, cloning unknown neighboring DNA, and library construction and screening...read more

Product Description: This volume focuses on newly emerging technologies that facilitate the isolation and characterization of genes. The detailed protocols will be useful to the seasoned professional and easily understood by the novice. The vast majority of methods are applic

Product Description: This book is one of four which together are considerably updated and totally revised texts that arose out of the very successful "DNA Cloning, Volume I-III" books in the Practical Approach series, edited by David Glover. The contents of this third volume are diverse and cover a variety of techniques for cloning and expressing DNA molecules...read more (view table of contents, read Amazon.com's description)

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Product Description: This is a manual which aims to give students a chance to explore the process and techniques of characterizing and purifying a protein, and of subsequent cloning of the associated gene. Each chapter emphasizes the process of discovery, and the strategies and rationale for each experiment...read more

Product Description: Expression systems are in widespread use for cloning specific genes, for synthesizing the encoded proteins for structural and functional analysis, and for large-scale preparation of proteins of commercial interest. Part of a four-volume set on DNA cloning, this book covers the main expression systems in current use...read more

Product Description: Part of a four-volume set on DNA cloning, this particular book describes all of the necessary background and detailed laboratory methods for the core techniques used in every molecular biology laboratory.

Product Description: Introduction to Molecular Cloning Techniques is a concise summary of the basis principles and methods used in genetic engineering and is intended for students and research technicians involved in microbiology, molecular biology, genetics, bioengineering, biotechnology, and chemical engineering...read more (view table of contents, read Amazon.com's description)

Product Description: This manual is based on the experimental protocols for an intense, practical course on gene cloning and analysis held in Leicester during September 1985 and July 1986.